NASA has successfully launched 166 crewed flights. Three have ended in failure, causing the deaths of seventeen crewmembers in total: Apollo 1 (which never launched) killed three crew members in 1967, STS-51-L (the Challenger disaster) killed seven in 1986, and STS-107 (the Columbia disaster) killed seven more in 2003. Thus far, 163 missions were conducted without fatalities.
Reference: demo_17
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